Policy #18
Approved August 13, 2007
Effective date September 1, 2007
Conditioning and Quarantine Policy
References:
Guide for the Care and Use of Laboratory Animals, NRC, 1996
Animal Welfare Regulations (9CFR)
Policy:
1.) All vertebrate animal species, regardless of distance shipped, are to be allowed a minimum of seven full days to stabilize and acclimate to one SMPH animal housing facility and recover from shipping stress prior to utilization in any survival surgical procedure. Shorter periods must be approved in advance by the SMPH - IACUC (animal care and use committee) through scientific justification in an approved protocol or the SMPH Attending Veterinarian. The laboratory animal veterinarian may extend this conditioning period for any medical reason and may also in his or her professional judgment return, request replacement or deny use for medical reason(s) any animal(s) intended for use in a survival surgical protocol. A recorded signature of approval on the medical record form for all non-rodent species from an SMPH clinical veterinarian is required prior to the surgery being performed.
2.) Animals intended for use in SMPH - ACUC approved non-survival surgery or acute use protocols are not required to have a minimum conditioning period. However, it is strongly recommended that at least 48 hours for the purpose of rehydration, stabilization and acclimation to the new environment be allowed prior to utilization in any research protocol.
3.) All vertebrate animals of all species will be housed in animal rooms based on vendor source, health monitoring and disease surveillance history.
- Only one species will be housed in any one room.
- Only animals purchased from approved vendors1 will be housed in SPF* (specific pathogen free) facilities and preferably only one vendor per room when feasible.
- Animals obtained from non- approved vendors and other universities or research institutions will be suspected to be non SPF and all mice and rats will be scheduled for rederivation by embryo transfer2 prior to introduction into SPF facilities.
- All Non-Human Primates will be quarantined upon arrival in accordance with LAR policy #203.
*SPF at SMPH is defined as the current Health Assessment Plus serology panel and fecal PCR testing for all Helicobacter species from Charles River Laboratories or the equivalent panel from other commercial laboratories (e.g. the Comprehensive Plus panel from the Research Animal Diagnostic laboratory (RADIL). The definition of SPF will upgrade as this serology panel upgrades. Please the rodent SPF list attached for more specific details.
4.) Any exceptions to this policy must be included and scientifically justified in an animal use protocol approved by the SMPH- IACUC (animal care and use committee).
1Approved vendors are commercial vendors which the SMPH veterinarians have identified as having the highest standards available with respect to being pathogen free and general health. (e.g. Charles River Laboratories, Taconic, Jackson Laboratories, Harlan,)
2Rederivation by embryo transfer should be arranged with the Transgenic Animal Facility. Contact Anne Griep at phone: 2-8988 or email: aegriep@wisc.edu
Role of Animal Care and Use Committee
The ACUC shall review proposed protocols for conformity to this policy and enforce compliance.
Role of Veterinary Staff and LAR Staff
The research animal veterinary staff and LAR staff will work cooperatively to ensure this policy is followed, and will report deviations to the ACUC.
Role of Investigator
The Investigator is responsible for adhering to this policy, and assuring all personnel working with animals under the Investigator’s ACUC-approved animal use protocols adhere to this policy.
School of Medicine and Public Health Rodent SPF
(Specific Pathogen Free) List
University of Wisconsin – Madison
Mouse Specific Pathogen Free –List for August 2007 –present
1.) Murine Norovirus (MNV)
2.) Mouse Parvovirus -1 (MPV-1)
3.) Mouse Parvovirus -2 (MPV-2)
4.) Minute virus (MVM)
5.) Non-Structural protein-1 (NS-1)
6.) Mouse Hepatitis Virus (MHV)
7.) Theilers’s Murine Encephalomyelitis Virus (TMEV)(GDVII)
8.) Mouse Rota virus (EDIM)
10.) Sendai virus (SEND)
11.) Pneumonia Virus of Mice (PVM)
12.) Reovirus (REO)
13.) Lymphocytic Choriomeningitis Virus (LCMV)
14.) Mouse Adenovirus (MAV)
15.) Ectromelia (ECTRO)
16.) K virus (K)
17.) Polyoma Virus (POLY)
18.) Mouse Thymic Virus (MTLV)
19.) Mouse Cytomegalovirus (MCMV)
20.) Hantavirus (HANT)
21.) Lactate Dehydrogenase - Elevating Virus (LDV) (LDHV)
22.) Encephalitozoon cuniculi (ECUN)
23.) Cilia –associated respiratory bacillus (CARB)
24.) Mycoplasma pulmonis (MPUL)
25.) Helicobacter Genus (H. hepaticus, H. bilis)
26.) Clostridium piliforme (Tyzzer’s disease)
27.) Pasteurella pneumotropica
All Endoparasites: pinworms, tapeworms
All Ectoparasites: mites
Rat Specific Pathogen Free – List for July 2007 – present
1.) Rat parvovirus (RPV)
2.) Toolan’s H-1 Virus (H-1)
3.) Kilham’s rat Virus (KRV)
4.) Rat Minute Virus (RMV)
5.) Non-Structural protein-1 (NS-1)
6.) Sialodacryoadenitis Virus (RCV)(SDAV)
7.) Rat Theilovirus (RTV)
8.) Sendai Virus (SEND)
9.) Pneumonia Virus of Mice (PVM)
10.) Reovirus (REO)
11.) Mycoplasma pulmonis (MPUL)
12.) Lymphocytic Choriomeningitis Virus (LCMV)
13.) Mouse Adenovirus (MAV)
14.) Theilers’s Murine Encephalomyelitis Virus (TMEV)
15.) Hantavirus (HANT)
16.) Encephalitozoon cuniculi (ECUN)
17.) Cilia –associated respiratory bacillus (CARB)
18.) Helicobacter spp.
19.) Clostridium piliforme (Tyzzer’s disease)
20.) Pasteurella pneumotropica
All Endoparasites: pinworms, tapeworms
All Ectoparasites: mites, lice
Contact Information:
James Goodrich, DVM, diplomate ACLAM
Phone: (608) 263-0664
Email: jagoodrich@wisc.edu
Attending Veterinarian
School of Medicine and Public Health
University of Wisconsin – Madison
Madison, WI 53706
